A study of 2,4,6-trinitrobenzenesulfonic acid for automated amino acid chromatography.
نویسنده
چکیده
The reaction of 2,4,6-trinitrobenzenesulfonic acid (TNBS) with amino acids was investigated by a static system to develop optimum conditions for application to automated amino acid chromatography. TNBS was found to be a very simple and sensitive reagent, easier to use than ninhydrin. The reaction occurs quite rapidly at room temperature, eliminating the requirement of a heating bath. The major disadvantage is a much lower sensitivity to the imino acid proline than to the amino acids.
منابع مشابه
An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines.
The use of 2,4,6-trinitrobenzenesulfonic acid (TNBS) as a reagent for determining the concentrations of amines has been widely accepted (1-3) since its introduction in 1960 by Satake et al. (4). The original procedure has since been modified by Mokrasch (5) to permit the determination of amines, amino acids, and proteins in mixtures. In both procedures the trinitrophenylation reaction is follow...
متن کاملAssessment of Serum Amino Acid Chromatography in Children with Inflammatory Bowel Diseases
Background: Given the important role of amino acids in regulating many metabolic pathways of the body and considering the scarcity of markers for the diagnosis of inflammatory bowel disease (IBD) and its differentiation, we aimed to investigate the status of serum amino acids chromatography in children with IBD. Materials and Methods This case-control study was conducted among children with pri...
متن کاملCellulose sulfuric acid catalyzed multicomponent reaction for efficient synthesis of pyrimido and pyrazolo[4,5-b]quinolines under solvent-free conditions
Cellulose sulfuric acid was used as an efficient biopolymer-based catalyst for the synthesis of tetrahydropyrimido[4,5-b]quinoline-2,4,6-triones and hexahydro-2H-pyrazolo[5,4-b]quinoline-6-ones via three component reaction of aldehyde, 5,5-dimethyl-1,3-cyclohexadione and 6-amino-1,3-dimethyluracil or 5-amino-3-methyl-1-phenypyrazole under solvent-free conditions at 90 oC. The major advantages o...
متن کاملCellulose sulfuric acid catalyzed multicomponent reaction for efficient synthesis of pyrimido and pyrazolo[4,5-b]quinolines under solvent-free conditions
Cellulose sulfuric acid was used as an efficient biopolymer-based catalyst for the synthesis of tetrahydropyrimido[4,5-b]quinoline-2,4,6-triones and hexahydro-2H-pyrazolo[5,4-b]quinoline-6-ones via three component reaction of aldehyde, 5,5-dimethyl-1,3-cyclohexadione and 6-amino-1,3-dimethyluracil or 5-amino-3-methyl-1-phenypyrazole under solvent-free conditions at 90 oC. The major advantages o...
متن کاملPreliminary Report of NAD+-Dependent Amino Acid Dehydrogenase Producing Bacteria Isolated from Soil
Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 14 10 شماره
صفحات -
تاریخ انتشار 1968